INTRODUCTION

Cell Counting Kit (CCK) utilizes the highly water-soluble tetrazolium salt  [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monosodium salt] to produce a water-soluble formazan dye upon reduction in the presence of an electron carrier.

Cell Counting Kit is a one-bottle solution; no premixing of components is required. Cell Counting Kit, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. CCK is reduced by dehydrogenases in cells to give a yellow colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

Feature

• One-bottle, ready-to-use solution.
• No organic solvents or isotopes required.
• No harvesting, no washing and no solubilization steps.
• More sensitive than MTT, XTT, MTS or WST-1.
• Low toxicity.

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