KOT – TUBULAR COILS
EXAMPLE APPLICATION AREAS
Biotech KOT reactors can be applied as a delay line in chromatographic separations. One example being the parallel coupling of a MS detector and a fraction collector in preparative separations (see figure below). The delay time induced by the KOT allows the detector to analyse the eluate and make intelligent decisions of when a new fraction is going to be initiated, without loss of chromatographic efficiency.

The Biotech KOT reactors can also be used in post-column reaction detection in HPLC (see figure below), using both room temperature and heated chemistries. In this set-up the KOT also ensure thorough mixing between the column effluent and the added reagents. Typical reactions are the production of fluorescent products from reactions between eluted compounds and reagents without native fluorescence.

KNITTED OPEN TUBULAR REACTION DELAY AND MIXING COILS

PRESERVED CHROMATOGRAPHIC PEAK SHAPE
The Biotech knitted open tubular (KOT) reactors are made from tubing which has been knitted into a tortuous path to force the fast moving centre of the liquid stream to mix radially with the slower moving boundary liquid layer, thereby minimizing the axial dispersion.

The shape of a chromatographic peak entering the reactor is thus preserved very efficiently. To ensure a stable radial mixing within the KOT, a linear flow rate of 10 cm/s or more is recommended. The KOT reactor is usually the most optimal delay element for use in analytical flow systems, and is typically inserted in the flow path to create a delay line, so that a reaction or other event that requires a certain time can take place.

Different delay or reaction times are accomplished by changing the inner diameter and length of the KOT, taking the flow rate through the KOT into consideration.
 

At Biotech Fluidics, our aim is to help you make your analytical work more effective and reliable. For more than 15 years we have developed and delivered innovative laboratory products to leading laboratories, instrument manufacturers and distributors across the world. We specialize in chromatography instruments, detectors and consumables plus finding solutions to issues with gases and bubbles in fluidic systems. By using our advanced degassers, labs can optimize the performance of their analytical instruments and chromatographic equipment. Our degassing solutions save the industry both time and money by avoiding production disruptions caused by bubbles in the fluidic lines. To support long-term relationships with our customers, it is important for us to take care of the aftermarket effectively. We have therefore built an organization of service and support. In addition, we offer fast delivery of spare parts directly from the company’s stock.