Purification is recommended for all oligos greater than 40 bases in length and for many modified oligos. For demanding applications such as multiplex PCR, cloning, mutagenesis or antisense/RNAi methods, additional purification will significantly improve oligonucleotide performance. Purified oligos up to 60 bases with a purity guarantee receive QC by capillary electrophoresis (CE). The traces are available online in your account order history.

Guaranteed yields for purified, unmodified oligos 20 to 50 bases in length. Purity and yield guarantees may vary due to oligo length, modifications, or sequence composition.

 

	100 nmole	250 nmole	1 µmole	5 µmole	10 µmole
PAGE Purification	1 OD	2 ODs	10 ODs	50 ODs	100 ODs
HPLC Purification	1 OD	4 ODs	20 ODs	100 ODs	200 ODs
IE-HPLC Purification	1 OD	4 ODs	20 ODs	100 ODs	200 ODs
RNase-Free HPLC Purification	1 OD	4 ODs	20 ODs	100 ODs	200 ODs
Dual HPLC Purification	1 OD	2 ODs	10 ODs	50 ODs	100 ODs
Dual PAGE & HPLC	0.5 OD	1 OD	5 ODs	25 ODs	50 ODs

 

PAGE Purification

PAGE obtains extremely high purity, especially for unmodified oligos and Ultramer® oligos. PAGE purification is strongly recommended for oligos over 60 bases in length. For unmodified oligos, purity of >90% is routinely achieved. Purity may vary due to oligo length, modifications, or sequence composition.

 

HPLC Purification

HPLC is well suited to purify any modified oligo or unmodified sequences up to 60 bases in length. Purity of > 85% is routinely achieved. Purity may vary due to oligo length, modifications, or sequence composition.

Ion-exchange HPLC (IE-HPLC) is available to purify long DNA oligos. For unmodified oligos, purity of > 85% is routinely achieved. Purity may vary due to oligo length, modifications, or sequence composition.

For the most demanding applications, IDT offers Dual HPLC Purification and Dual PAGE and HPLC Purification. These methods result in oligos of the highest possible purity. Dual HPLC purification is used to purify Dual‑Labeled Probes and Molecular Beacons with NHS Ester modifications. Purity may vary due to oligo length, modifications, or sequence composition.

RNase-Free HPLC Purification

Originally developed for isolating RNA oligos, RNase-Free HPLC Purification has been extended to DNA oligos for use when there is a high sensitivity to ribonucleases. RNase-Free HPLC purification is carefully performed in an RNase‑free environment; reagents, equipment and lab surfaces are monitored for RNase contamination using the RNaseAlert® system.

Guaranteed yields for purified, unmodified oligos 20 to 50 bases in length. Purity and yield guarantees may vary due to oligo length, modifications, or sequence composition.

About IDT

Integrated DNA Technologies (IDT) is a leader in manufacturing and developing products for the research and diagnostics life science market. IDT serves the areas of academic research, biotechnology, and pharmaceutical development. IDT was founded by Dr Joseph Walder in 1987. Since then, its development has been guided by an uncompromising approach to quality, a belief in the value of good service, and a determination to minimize consumer costs.

Serving over 80,000 life sciences researchers, IDT is widely recognized as the industry leader in custom oligonucleotides due to its capabilities in:

Analytical Sophistication—IDT pioneered the use of high throughput quality control (QC) methods and is the only oligonucleotide manufacturer to offer purity guarantees and 100% QC. Every oligonucleotide is analyzed by mass spectrometry and purified oligonucleotides receive further analysis by CE and HPLC.
Design Engineering—IDT maintains an engineering division dedicated to advancing synthesis, processing technology, and automation. An in-house machine shop provides rapid prototyping and custom part design/control.
Customer Support—IDT received over 100,000 calls last year with an average wait time of only 8 seconds.
Reagent and Input Control—IDT receives all solvents in large bulk containers, runs QC on all incoming materials, and performs bulk reagent formulation and functional QC on all reagents.
IDT has over 700 employees serving our worldwide customer base from headquarters in Coralville, Iowa, and facilities in San Diego, California; Leuven, Belgium; and Singapore.