Mass Spectrometry and Capillary Electrophoresis Data—Available FREE on the Web!
- Log into the IDT Ordering System.
- Go to Past Order Status.
- Select the order containing the purified oligos whose mass spectrometry or capillary electrophoresis (CE) data you want to view.
- To view the mass spectrometry or CE trace of a particular item, click the Mass Spectrometry or CE button next to the item.
Mass Spectrometry
All oligos synthesized at IDT are analyzed by electrospray ionization (ESI) mass spectrometry for quality control, ensuring that only the highest quality products are shipped.
Mass Spectrometry Analysis
Capillary Electrophoresis
CE provides quantitative analysis of micropurity. Nanoliter samples of oligonucleotides are electrokinetically injected into a fused silica capillary and filled with a denaturing gel under an electric potential. The oligonucleotides move through the gel matrix and are separated according to length. Absorbance at 260 nm is detected as the oligo passes through a window in the capillary. The resulting electropherogram provides the percent purity of the full-length product as well as truncated products.
CE Analysis
Analytical HPLC
HPLC is a universally accepted method of sample quality assessment. Both reverse-phase and ion-exchange methods are used at IDT to assess oligonucleotide product purity. These methods are used for purified oligos whose composition is incompatible with CE technology.
HPLC Analysis
For more information on mass spectrometry or CE traces, please contact Technical Support by phone at 1-800-328-2661 (USA/Canada), +32 (0) 16 28 22 60 (Europe), +65 6775 9187 (Asia Pacific) or by email attechsupport@idtdna.com.
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About IDT
Integrated DNA Technologies (IDT) is a leader in manufacturing and developing products for the research and diagnostics life science market. IDT serves the areas of academic research, biotechnology, and pharmaceutical development. IDT was founded by Dr Joseph Walder in 1987. Since then, its development has been guided by an uncompromising approach to quality, a belief in the value of good service, and a determination to minimize consumer costs.
Serving over 80,000 life sciences researchers, IDT is widely recognized as the industry leader in custom oligonucleotides due to its capabilities in:
Analytical Sophistication—IDT pioneered the use of high throughput quality control (QC) methods and is the only oligonucleotide manufacturer to offer purity guarantees and 100% QC. Every oligonucleotide is analyzed by mass spectrometry and purified oligonucleotides receive further analysis by CE and HPLC.
Design Engineering—IDT maintains an engineering division dedicated to advancing synthesis, processing technology, and automation. An in-house machine shop provides rapid prototyping and custom part design/control.
Customer Support—IDT received over 100,000 calls last year with an average wait time of only 8 seconds.
Reagent and Input Control—IDT receives all solvents in large bulk containers, runs QC on all incoming materials, and performs bulk reagent formulation and functional QC on all reagents.
IDT has over 700 employees serving our worldwide customer base from headquarters in Coralville, Iowa, and facilities in San Diego, California; Leuven, Belgium; and Singapore.
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