Description:
This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.
5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.Use in conjunction with lysis buffer to detect samples quickly and easily, with high sensitivity, specificity, and stability.
Specification
|
Kit composition
(20 μl qPCR reaction system)
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DRT-01011
|
DRT-01012
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Component
|
|
200 T
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1000 T
|
|
Part I
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Buffer CL
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4 ml
|
20 ml
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Cell Lysis
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|
Foregene Protease Plus II
|
80 μl
|
400 μl
|
|
Buffer ST
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400 μl
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1 ml × 2
|
|
Part II
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DNA Eraser
|
80 μl
|
400 μl
|
|
5× Direct RT Mix
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160 μl
|
800 μl
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RT
|
|
2× Direct qPCR Mix-SYBR
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1 ml × 2
|
1.7 ml × 6
|
qPCR
|
|
50× ROX Reference Dye
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40 μl
|
200 μl
|
|
RNase-Free ddH2O
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1.7 ml
|
10 ml
|
-
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|
Manual
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1 serving
|
1 serving
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1 serving
|
*: Cell Lysis, RT and qPCR components can be purchased separately. For details, see Appendix 1 of the full version of the manual (PAGE 13).
Features
- Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.
- The sample demand is small, as low as 10 cells can be tested.
- High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.
- DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.
- Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.
Kit application
- Scope of application: cultured cells.
- RNA released by sample lysis: only applicable to the RT-qPCR template of this kit.
- The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.
Transport and storage conditions
Transportation conditions: The whole process is transported in a low-temperature ice box to ensure that the kit is in a state of <4 °C.
Storage conditions: Part I of the kit is stored at 4 °C; Part II is stored at -20°C.
bio-equip.cn
Foregene Co., Ltd. is specialized in the molecular biology lab reagents, IVD raw materials, IVD Kits, and related products and services.Foregene is integrated in R&D, manufacturing and marketing together .
Established in 2011, Foregene has owned the world-class Direct PCR Platform,DNA-Only,RNA double spin column Isolation Platform. The main products include Direct PCR reagents, DNA/RNA isolation kits, PCR Master Mix, Genotyping kits, FISH Probe Kits, IVD Raw Materials, various IVD kits, and Lab Equipments. Foregene can provide the whole solution of IVD products based on our technology Platforms.
Becuse of the world-class leading technology, we are popular in domestic and overseas markets.Foregene’s high quality products are widely used in hospitals, universities, national research institutions, medical laboratories,CRO companies, bio-pharm companies, etc.
Looking forward to Cooperate with you.
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