Microscale thermophoresis (MST) is a technique for biophysical analysis of biomolecular interactions. MST is based on the detection of temperature induced target fluorescence changes as a function of the concentration of non-fluorescent ligands. MST can be used with almost all kinds of target molecules, if they are fluorescent or can be labeled with fluorescent dyes. The diversity of ligands is greater. The fluorescent target molecule keeps a constant concentration, and the non-fluorescent ligand molecule titrates in a large concentration range.

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