Multi-protein complexes play important roles in a wide range of cellular processes, including transcription, translation, RNA splicing, signaling cascades, protein trafficking and degradation. With the deepening of research, scientists realize that the function of a protein depends not only on its own physicochemical properties, but also on its interactions with its partners, and the characteristics of them, so more and more studies begin to focus on protein complex's structure and function. Protein complexes have shown important roles in the biotechnology industry, metabolic engineering and even in the biopharmaceutical therapy, and the strategies of reconstructing multi-proteins through protein co-expression has become widely used methods.

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