Profacgen also improved the phage-assisted continuous directed evolution system and method, which included phages carrying target genes to be evolved, multiple helper plasmids supporting the proliferation of different phages before and after evolution, and plasmids inducing mutations. The placement of different Hp in different host bacteria can effectively improve the efficiency of phage proliferation and evolution, while avoiding the mutual interference between different genetic elements, which has good practicability and can be used for the directional evolution of a variety of genes.

https://www.profacgen.com/phage-assisted-continuous-evolution-pace.htm

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