Cat. No.: MUAES-01101 Size: 1×10⁶cells/vial

Description:
Strain 129 Mouse Embryonic Stem Cells are derived from the inner cell mass of strain 129 mice blastocyst (at 3.5 days post-coitus) and cultured on γ-ray irradiated mouse embryonic fibroblasts (MEF) (Cat. No.: MUIEF-01002) as feeder cells in Mouse Embryonic Stem Cell Growth Medium (Cat. No.: MUXES-90011).
Strain 129 Mouse Embryonic Stem Cells stably expressing GFP are established.
Cells are supplied in a cryovial containing 1×106cells.

Characterization of cells:
Strong capacity to self-renew.

Positive for alkaline phosphatase staining.
Ability to form tetatoma in severe combined immunodeficiency (SCID) mice.
Expression of OCT-4, SSEA-1 and nanog.
Displaying normal diploid karyotype (40, XY or 40, XX) during extensive subcultivation.
Ability to differentiate into cell types of all three germ layers in vitro and in vivo .

Quality control:
Cells are tested negative for bacteria, fungi, mold, HIV, hepatitis-B&C, and mycoplasma.

Delivery and storage:
Frozen cells are shipped on dry ice.
Upon receipt, if no dry ice is left in the package, thaw and use them immediately.
If there is dry ice left in the package, store it in liquid nitrogen immediately upon arrival until it is convenient to proceed to subculture.
When stored at the recommended storage conditions, components are stable up to the expiration date. Do not expose to elevated temperature.

Product warranty:
Cyagen warrants its cells only if suggested media are used and the recommended protocols are followed. Cryopreserved Strain 129 Mouse Embryonic Stem Cells/GFP are assured to be viable and functional when thawed and maintained properly.

Usage:
This product is for research use only, and not intended for human or animal diagnostic or therapeutic use.

Cyagen provides various kinds of human and animal embryonic and adult stem cells, genetically engineered cell lines and their culture media for scientific research and drug discovery purposes.

Cyagen provides services in transgenic animals including knockout mice, and stem cell based drug discovery.

Cyagen is striving to build innovative drug discovery platforms based on latest technologies in stem cell biology and molecular biology.