Features
• Thermostable reverse transcriptase 45°C to 55°C
• Proprietary hot-start technology for unrivalled detection of low copy   number templates
• Contains advanced RNase inhibitor
• High PCR yields under standard and fast PCR conditions
• Efficient specific amplification from complex templates including GC rich and AT rich sequences

PCRBIO 1-Step RT-PCR Kit uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and PCR in a single tube. The PCR is performed by PCRBIO Taq HS DNA Polymerase, resulting in robust RT-PCR performance.

“Hot-start” is a term used to describe the inactivation of a DNA polymerase until the initial activation step at 95°C. Inactivation below 65°C prevents primer-dimer formation and non-specific amplification allowing for specific amplification from low copy number target sequences. Our proprietary small-molecule hot-start technology offers improved specificity and sensitivity compared to other methods.

PCRBIO Taq HS DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO Taq HS DNA Polymerase can perform consistently well on a broad range of templates (including both GC and AT rich).

Cat Number		Product Name	Size	Price
: PB10.52-05		PCRBIO 1-Step RT-PCR Kit	50 reactions	[Contact us for a price]
: PB10.52-10		PCRBIO 1-Step RT-PCR Kit	100 reactions	[Contact us for a price]
: PB10.52-50		PCRBIO 1-Step RT-PCR Kit	500 reactions	[Contact us for a price]

PCR Biosystems offers a range of best-in-class kits and reagents for PCR and related technology. By combining our enhanced polymerases with highly-developed reaction buffers and novel hot-start chemistry PCR Biosystems is leading the development of PCR. Our PCR reagents have been developed to maximise yield and sensitivity in the simplest and most challenging of reactions.

Our portfolio covers a broad range of applications including, real-time PCR, endpoint PCR, high fidelity PCR, hot-start PCR, long PCR, PCR direct from crude samples and molecular diagnostic PCR.

All PCR Biosystems products have undergone comprehensive competitor product comparisons. On average we out perform all competitors in yield, specificity, sensitivity and speed. For more information on our technology click here.

PCR Biosystems produces, manufactures and distributes under a comprehensive quality management system, following ISO 9001:2008 standards.