As an experienced service provider in molecular discovery and research, Profacgen offers high-quality MDR1-MDCK permeability assay to simulate the process of drug from the intestinal lumen into the blood. Similar to the Caco-2 assay, our MDR1-MDCK permeation system uses a monolayer of MDCK cells as the filter membrane, and the cells are seeded on 24- or 96-well plates to form a confluent monolayer, compound transport across the monolayer is monitored over a 60-min period to determine penetration efficiency of compounds from apical to basolateral or basolateral to apical. Compounds are quantified by LC-MS/MS analysis based on peak area.

https://www.profacgen.com/madin-darby-canine-kidney-cell-line-mdck-permeability-assay.htm

Profacgen offers a full range of biopharmaceutical development and bio-manufacturing services that cover strategic consultancy, world-class process development, cGMP manufacturing, and analytical development.