Human Mesenchymal Stem Cell Growth Medium (HMSC-GM)

★ Technical Manual

     Human Mesenchymal Stem Cell Growth Medium (HMSC-GM)

★ Catalog NO: BW110011

★ Components

           465 ml of Basal Medium

             25 ml of Qualified Fetal Bovine Serum (FBS)

                5 ml of Human Mesenchymal Stem Cell Supplement (HMSCS)

                5 ml of penicillin/streptomycin solution (P/S)

★ Product Description

     Human Mesenchymal Stem Cell Growth Medium（HMSC-GM）contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and pre-selected fetal bovine serum (FBS) and other supplements. The product is suitable for culturing human mesenchymal stem cells isolated from bone marrow. The product is strictly aseptic without any contamination of virus or mycoplasma, and exhibits stable performance. By using this medium, human mesenchymal stem cells can be quickly expanded with minimal differentiation.

★ Instruction for use

1.  Prior to use, thaw the Qualified FBS and penicillin/streptomycin solution (P/S) overnight at 2 to 8 ℃.

2.  Add the above thawed solutions to the Basal Medium, gently swirl the bottle to ensure the mixture homogeneity.

3.  Thaw the Human Mesenchymal Stem Cell Supplement (HMSCS) in water bath (37℃) and then add it into the above mixture to make the complete medium.

4.  Store the complete medium in the dark at 4℃.

★ Product use

     This product is for laboratory research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

★ Storage condition

     Basal Medium should be stored at 4℃, while HMSCS, FBS and P/S solution should be stored in the dark at -20℃.

ArkOne Biotechnologies, Inc. is a global distributor of high quality biochemical reagents and lab research tools for protein chemistry, molecular biology, and cell biology including a series of DNA vectors, purified proteins of high quality, and media for cell culture. The pre-selected media are suitable for stem cell culture, especially for those mammalian stem cells isolated from bone marrow, umbilical cord, and adipose tissues. By using these media, cells can be quickly expanded with minimal differentiation.The serum media contain essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and pre-selected fetal bovine serum and other supplements. The serum-free media contain defined animal-free components and key cytokine supplements, targeting clinical applicaiton. We also provide packaging services for a variety of viruses including intergrative viruses and non-integrative viruses of high titer. Integrative viruses are suitable for rapid generation of stable cell lines. These viruses include retrovirus and classical lentivirus. Non-integrative viruses include modified lentivirus, adenovirus and adeno-associated viruses (AAV) with serotypes ranging from AAV2/1 to AAV2/9. Non-integrative viruses can serve as excellent options for transient transfection as well as for gene delivery treatment (http://).