ChIP Validated Antibody & Positive Primer Sets

Chromatrap^® ChIP-validated Antibodies can now be supplied with the correct matching primer sets to cover all aspects of ChIP validation.

•Reduce time spent searching databases and designing forward and reverse primers
•Purchase superior quality ChIP-validated antibodies with their matching high-quality primers
•Have confidence that the primers are tested with the exact antibody and gene target

The Chromatrap^® ChIP Validated Antibody Primer sets include a positive control antibody, a negative control antibody (non immune IgG) to evaluate non-specific binding and species-specific positive control qPCR primers.

ChIP Validated AR Antibody with Positive Primer Set
Product Code : 900019

Chromatrap^®’s ChIP Validated AR Antibody and Positive Primer Set provides a complete set of tools to assist with a successful ChIP assay. Including: AR antibody, control sheep IgG, and a positive primer set. The ChIP Validated AR Antibody and Positive Primer Set is not suitable for use with non-human species.

Background:

The androgen receptor (AR) is a steroid hormone receptor involved in the signalling of testosterone. It is a regulatory gene that controls gene expression through binding to androgen receptor elements (AREs). The AR is associated with both normal prostate and cancerous prostate cell growth at abnormal levels of the gene (Lamb et al, 2014, BJUI). Androgens are also heavily involved in the pathogenesis of endometrial cancer (D.A. Gibson et al, 2014, Endocr Relat Cancer). DHT (dihydrotestosterone) is a natural androgen hormone that can activate the androgen receptor (Mostaghel, 2014, IJBS).

A sheep IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.

The androgen receptor positive primer set recognises a protein-coding gene that is regulated by androgen.

•Applications: ChIP
•Source: Sheep
•Concentration: 1mg/ml
•Type: Monoclonal
•Size: 50μl
•Purification: Protein G (affinity purified)
•Specificity: Human
•Storage Conditions: The AR antibody and sheep IgG +4°C. The positive primer set -20°C

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