ChIP Validated Antibody Primer Sets with positive and negative gene targets

Chromatrap^® ChIP-validated Antibodies can now be supplied with the correct matching primer sets to cover all aspects of ChIP validation.

 •Reduce time spent searching databases and designing forward and reverse primers
 •Purchase superior quality ChIP-validated antibodies with their matching high-quality primers
 •Have confidence that the primers are tested with the exact antibody and gene target

Many common epigenetic targets of interest are now available as combined antibody & primer sets from Chromatrap^®.

ChIP Validated AR Antibody with Positive and Negative Primer Sets
Product Code : 900006

Chromatrap^®’s ChIP Validated AR Antibody with Positve and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: AR antibody, control sheep IgG, positive and negative primer sets. The ChIP Validated AR Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.

Background:

The androgen receptor (AR) is a steroid hormone receptor involved in the signalling of testosterone. It is a regulatory gene that controls gene expression through binding to androgen receptor elements (AREs). The AR is associated with both normal prostate and cancerous prostate cell growth at abnormal levels of the gene (Lamb et al, 2014, BJUI). Androgens are also heavily involved in the pathogenesis of endometrial cancer (D.A. Gibson et al, 2014, Endocr Relat Cancer). DHT (dihydrotestosterone) is a natural androgen hormone that can activate the androgen receptor (Mostaghel, 2014, IJBS).

A sheep IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.

The androgen receptor positive primer set recognises a protein-coding gene that is regulated by androgen. The negative gene target included recognises a gene that is not present at the androgen receptor.

 •Applications: ChIP
 •Concentration: 1mg/ml
 •Size: 50μl
 •Specificity: Human
 •Storage Conditions: The AR antibody and sheep IgG +4°C. Primer sets -20°C

Porvair Sciences, specialists in the manufacture of microplate products, serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, from sample preparation to high throughput screening via our global distributor network.

Our range includes vacuum manifolds, sealers, evaporators and microtiter plates in all popular styles; deep well and shallow well storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs. We also provide custom microplate products for life science research.

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