ChIP Validated Antibody Primer Sets with positive and negative gene targets

Chromatrap® ChIP-validated Antibodies can now be supplied with the correct matching primer sets to cover all aspects of ChIP validation.

•Reduce time spent searching databases and designing forward and reverse primers
•Purchase superior quality ChIP-validated antibodies with their matching high-quality primers
•Have confidence that the primers are tested with the exact antibody and gene target

Many common epigenetic targets of interest are now available as combined antibody & primer sets from Chromatrap®.

ChIP Validated RNA Pol II (S5) Antibody with Positive and Negative Primer Sets
Product Code : 900004

Chromatrap®’s ChIP Validated RNA Pol II (S5) Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: RNA Pol II (S5) antibody, control mouse IgG, positive and negative primer sets. The ChIP Validated RNA Pol II (S5) Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.

Background:

RNA polymerase II catalyses the transcription of DNA into mRNA and other small nuclear RNAs in eukaryotes. It contains a CTD consisting of conserved heptapeptide repeats. Phosphorylation occurs at serine and therine residues located in the CTD repeats to activate the RNA pol II. RNA pol II binds to several transcription factors in order to initiate transcription and therefore serves as an abundant antibody target for ChIP. This antibody recognises the phosphorylated (serine 5) form of RNA Pol II in humans.

A mouse IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.

The positive primer set included in this Antibody Primer Set recognises a gene associated with active transcription due to the role of RNA Pol II (S5) in catalysing transcription. The negative gene target included recognises a gene that is associated with repressive transcription.

 •Applications: ChIP
 •Source: Mouse
 •Concentration:95mg/ml
 •Type: Monoclonal
 •Size: 50μl
 •Purification: Protein A (affinity purified)
 •Specificity: Human
 •Storage Conditions: -20°C

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