ChIP Validated Antibody Primer Sets with positive and negative gene targets

Chromatrap^® ChIP-validated Antibodies can now be supplied with the correct matching primer sets to cover all aspects of ChIP validation.

•Reduce time spent searching databases and designing forward and reverse primers
•Purchase superior quality ChIP-validated antibodies with their matching high-quality primers
•Have confidence that the primers are tested with the exact antibody and gene target
Many common epigenetic targets of interest are now available as combined antibody & primer sets from Chromatrap^®.

Many common epigenetic targets of interest are now available as combined antibody & primer sets from Chromatrap^®.

ChIP Validated 5mC Antibody with Positive and Negative Primer Sets
Product Code : 900000

Chromatrap^®’s MeDIP Validated 5mC Antibody with Positive and Negative Primer Set provides a complete set of tools to assist with a successful MeDIP assay. Including: 5mC antibody, control mouse IgG, positive and negative primer sets. The MeDIP validated 5mC Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.

Background:

5mC (5-Methylcytosine) is a region of DNA that has been epigenetically modified through the addition of a methyl group at the 5th carbon position of cytosine. DNA methylation is a repressive histone modification that is associated with switching off gene expression. This methylation generally occurs at CpG islands and is also associated with genomic imprinting. Aberrant DNA methylation is linked to disease including many cancers.

A mouse IgG is included in this Antibody Primer Set as a negative control for the MeDIP experiment.

The positive primer set included in this Antibody Primer Set recognises an imprinted gene, a source of hypermethylation. It is therefore an appropriate target for the 5mC antibody. The negative gene target included recognises a hypomethylated, protein-coding gene.

•Applications: MeDIP
•Source: Mouse
•Concentration: 2mg/ml
•Type: Monoclonal
•Size: 50μl
•Purification: Protein G (affinity purified)
•Specificity: Human, mouse
•Storage Conditions:-20°C

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