Human Tumor Necrosis Factor α(TNF-α) ELISA Kit,specification,price,image-Bio-Equip in China

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Sample Types Validated:Serum, blood plasma,Saliva, Urine, and other related tissue Liquid.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Please carefully read this instruction before using. This ELISA kit is based on the principle of double-antibody sandwich technique to detect Human Tumor necrosis factor α(TNF-α). Be used only for research purposes, not be used for medical diagnosis..

Intended Use

This kit is used to assay the Tumor necrosis factor α(TNF-α)in the sample of Human ’s serum, blood plasma, and other related tissue Liquid.

Test principle

The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Tumor necrosis factor α(TNF-α)in samples. Add Tumor necrosis factor α(TNF-α) to monoclonal antibody Enzyme well which is pre-coated with Human Tumor necrosis factor α(TNF-α)monoclonal antibody, incubation; then, add Tumor necrosis factor α(TNF-α)antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concentration of the Human Substance Tumor necrosis factor α(TNF-α)of sample were positively correlated.

Materials supplied in the Test Kit

1	Standard(960ng/L)	0.5ml	7	Chromogen Solution A	6ml
2	Standard diluent	3ml	8	Chromogen Solution B	6ml
3	Microelisa Stripplate	12w×8s	9	Stop Solution	6ml
4	Str- HRP-Conjugate Reagent	6ml	10	Instruction	1
5	30×wash solution	20ml	11	Closure plate membrane	2
6	Biotin-TNF-α Ab	1ml	12	Sealed bags	1

Materials required but not supplied

1. 37 ℃ incubator 2. Standard Enzyme reader

3. Precision pipettes and Disposable pipette tips 4. Distilled water

5. Disposable tubes for sample dilution 6. Absorbent paper

Important Notes

1. Beening taken out from the 2-8℃ environment, the kit should be balanced 30 minutes in the ambient temperature then use. If the Coated plates of Enzyme haven’t been used up after opened, the remaining plates should be stored in Sealed bag.

2. For each step, add Sample with sample injector which should be calibrated frequently, in order to avoid unnecessary experimental tolerance.

3. he operation shall be carried out accordance to the instructions strictly. And test results must be based on the readings of the Enzyme reader.

4. In order to avoid cross-contamination, it is forbidden to re-use the suction head and seal plate membrane in your hands.

5. All samples, washing buffer and each kind of reject should according to infective material process.

6. The idle agents shall be put up or covered. Do not use reagent with different batches. And use them before expired date.

7. The substrate B is light-sensitive. Prolonged exposure to light is forbidden.

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Email: rebecca@spanbio.com

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