1.  Principle
This test kit is based on the competitive enzyme immunoassay for the detection of β-Lactams in the tissue, liver, honey, intestine, shrimp, fish, milk, etc. The coupling antigens are pre-coated on the micro-well stripes. The β-Lactams in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the β-Lactams receptors. After the addition of the antibodies and enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with β-Lactams concentration in the sample. This value is compared to the standard curve and the content of the corresponding β-Lactams is subsequently obtained.
 
2.  Technical specifications
Sensitivity : 0.10ppb     Detect time: Approx. 75 min
Detection limit:
Tissue, liver, honey, shrimp, fish, milk ........... 2ppb
 
Recovery rate:
Tissue, liver, honey, shrimp, fish, milk....... 85±10%
 
Cross-reaction rate:
   Penicillin .......................100%
Ampicillin...................... 90 %
Amoxicillin.................... 80 %
Nafcillin……………… 110%
Amoxicillin …………… 80%
Piperacillin……………. 80%
Azlocillin...................... 90%
Cloxacillin.....................75%
Dicloxacillin...................50%
Oxacillin ....................... 50%
Metampicillin..................50%
Metampicillin..................30%
Carbenicillin....................10%
Ticarcillin...................... 10%
Cephalosporins..............< 0.01%
 

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Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.

Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.

Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.

Ecalbio has an enthusiastic and creative R&D team, consisting with a group of experienced technicians in lateral flow immunoassay field, and set up long relationship with first-class institutes,universities,academies, to provide excellent service for human healthy and earth.

Ecalbio are committed to saving lives by ensuring individuals worldwide have access to timely and correct diagnosis. Our products are used in over 100 countries worldwide by:
　Government Laboratories
　Food industry Laboratories
　Institutes
　Veterinary Clinics
　Pharmaceutical Industry.

Ecalbio welcomes potential partners and distributors to explorebusiness relationships with all customer in the worldwide, focused on scie-tech, integrity, quality and service for the customers.

ECALBIO CO.,LTD

HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com