intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Glycogen Phosphorylase, Liver (PYGL) concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

sample preparation

1. DL Sci&Tech Development Co.Ltd is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.

2. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.

3.If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.

4. Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts from certain chemicals.

5. Due to the possibility of mismatching between antigen from other origin and antibody used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.

6. Influenced by the factors including cell viability, cell number or sampling time, samples from cell culture supernatant may not be detected by the kit.

7. Fresh samples without long time storage is recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.

calculation of the result

Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Create a standard curve on log-log graph paper, with  Mouse Glycogen Phosphorylase, Liver (PYGL)  concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

Note

The Stop Solution suggested for use with this kit is an acid solution.Wear eye,hand,face and clothing protection when using this material.

Wuxi Donglin Sci & Tech Development Co.,Ltd., a manufacturer and supplier of biological reagents for life science, is located in the scenic area beside Taihu Lake. At the inception in 1997, our company focused on providing OEM service of ELISA kits across the world. In 2013, we launched our own brand Dldevelop.

We mainly specialize in manufacturing ELISA kits, distributed to Asia, Oceania, Africa, Europe and America. Headquartered in Wuxi, China, we have an overseas branch in Canada, a domestic branch in Shanghai, China.

Selected raw materials and state-of-the- art technologies help ensure the superior quality of our products with a one-year shelf life, which is six months longer than other manufacturers.
We have more than 6000 ElISA kits available. With the advance of our technology , more kinds of and more specific ELISA kits will be developed and manufactured to meet scientists and researchers’ need.

we strictly obey national and international QC requirements. Our company is a ISO9001:2008 and CE certificated enterprise.

Based on our strong innovative R&D teams, we are always committed to providing global scientists and researchers with the high quality ELISA kits and the best customer experience.