ProClin300 Preservatives

To solve the problem of too short shelf life of storing bio-specimen, ShineGene Co. has developed a new generation of high effective preservatives, ProClin series, including ProClin150, 200, 300, 5000. These ProClin preservatives are highly effective biocides for controlling microorganisms in reagents and products intended for in vitro diagnostic use. Used at concentrations as low as 0.02%, these preservatives eradicate bacteria, fungi, and yeast for extended periods of time. Both preservatives offer excellent compatibility and stability with most enzyme systems, as well as low toxicity, so ProClin preservatives are an excellent choice for replacing thimerosal, sodium azide, and gentamicin preservatives.

advantages of ProClin preservatives:
1. Broad-spectrum antimicrobial activity controls of bacteria, yeast, and fungi, and fast reaction speed.
2. Compatible with key enzymes, and do not inhibit antibody/ enzyme activity.
3. High effectiveness in low concentration. 6~20ppm ProClin300 can get a good preservative effect.
4. Effective at pH 2-8.5, and excellent stability. By Lab test, only 3% of the preservative contents will lose after 2 years of storage at 25C.
5. Less toxic than thimerosal
6. Can be mixed with water at any ratio.

The mechanism of Action of ProClin preservatives.
The active components in ProClin preservative are two isothiazolones: 2-methyl-4-isothiazolin-3-one and 5-chloro-2-methyl-4- isothiazolin-3-one. These active components have a unique mechanism of action that both inhibits microbe growth and causes cell death. Within minutes after contacting a microorganism, the active components penetrate the cell membrane and inhibit specific enzymes in the cell (figure 1). The target enzymes are within the central metabolic cycle of the cell, and ProClin preservatives aims at the 4 sites of the Krebs cycle (pyruvate dehydrogenase, a-ketoglutarate dehydrogenase, succinate dehydrogenase, NADH dehydrogenase) (figure 2), inhibiting metabolism, macromolecule synthesis, and causing intracellular energy levels to decline rapidly. With energy production disrupted, the cell can no longer synthesize chemicals for routine operation or repair. Ultimately the cell dies. Since most of the bacteria and fungi undergo at least part of the KREBS cycle, ProClin preservatives have a wide application scope. It is also proved that ProClin preservatives possess multiple pathways, which may reduce the likelihood of resistance development. With stopping of KREBS cycle, the cell’s ability of generating energy (use ATP as an index, figure 3) and activity materials such enzymes has declined rapidly. But it will cause little harm to human or animals because of its low concentration usage.

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Shanghai ShineGene Molecular Biotech,Inc.
Floor 2,Building A,328#, Wuhe Road,
Minhang District,Shanghai,201109,China
Tel: +86-21-54460832
Fax: +86-21-54460831
Web: http://www.synthesisgene.com
www.shinegene.org.cn/english
E-mail: master@shinegene.org.cn

ShineGene Bio-Technologies, Inc., located in Shanghai, P.R. China, is a bio-company specialized in providing bio- technical services including Gene Synthesis,Peptide Synthesis,Real Time PCR, Fluorogenic Probe Design,Fluorogenic Probe Label,HRP Enzyme Stabilizer,X-gal,IPTG,ProClin300etc. and supplying reagents such as Hotstart Taq polymerase, dNTPs, qPCR mastermix,one step qPCR kits,First Strand cDNA Synthesis Kits,etc. ShineGene also undertake some OEM or OED orders as well as production task.ShineGene works at the cutting edge of development in PCR, quantitative real-time PCR, transposon and RNA technologies. ShineGenes mission is to provide new tools of excellent quality and extreme purity to the scientific community. Therefore research and development is a continuous process in our company.

http://www.synthesisgene.com
http://www.shinegene.org.cn/englsih