• For the quantitative determination of Human E1S
• Detection of species: Human
• Detection medium: serum, plasma, Urine, tissue samples, cell culture supernates

 

Principle of test

The kit is for the quantitative level of E1S in the sample, adopt purified Human E1S to microtiter plate, make solid-phase antibody, then add E1S to wells, Combine E1S antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of E1S in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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