The recombinant human Leptin protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human Leptin (NP_000221.1), (Val 22-Cys 167) was expressed with an additional Met.

The recombinant human leptin consists of 147 amino acids and predicts a molecular mass of 16 kDa. The apparent molecular mass of rhLEP is approximately 13 kDa in SDS-PAGE under reducing conditions.

Human Leptin protein measured by its binding ability in a functional ELISA .
1. Immobilized human Leptin at 1.25 μg/ml (100 μl/well) can bind human Leptin receptor Fc chimera with a linear range of 0.032-4.0 μg/ml.
2. Immobilized human Leptin at 5 μg/ml (100 μl/well) can bind human Leptin receptor his with a linear range of 0.032-4.0 μg/ml.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.