The recombinant mouse TNF-alpha protein is expressed in E. coli, whose amino acid sequence is derived from soluble form of mouse TNF-α (NP_038721.1), (Leu 80-Leu 235) was expressed, with an initial Met at the C-terminus.

The recombinant mouse TNF-α consists of 157 amino acids and migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.

Mouse TNF-alpha protein measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.04-0.08 ng/mL .

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