The recombinant human IL-4 protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human IL4 isoform 1 (P05112-1), (His 25-Ser 153) was expressed and purified, with an initial Met.

The recombinant human IL4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15 kDa as predicted.

Human IL-4 protein measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 × 10E4 IU/μg.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.