The recombinant human IFN-gamma protein is expressed in CHO Stable Cells, whose amino acid sequence is derived from human γ-IFN (NP_000610.2), (Met 1-Gln 166) was expressed and purified.

The secreted recombinant human γ-IFN consists of 143 amino acids and has a predicted molecular mass of 16.7 kDa. γ-IFN migrates as two bands with apparent molecular mass of 21&25 kDa probably due to different glycosylation.

Human IFN-gamma protein measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV) . The EC50 for this effect is typically 0.1-0.5 ng/mL.

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