The recombinant human SCF (aa 1-189) protein, His Tag is expressed in Baculovirus-Insect Cells, amino acid sequence derived from amino acid (Met 1-Ala 189) of human SCF (P21583-1) extracellular domain was expressed, with a polyhistidine tag at the C-terminus.

The secreted recombinant human SCF (aa 1-189) consists of 175 amino acids and predicts a molecular mass of 19.9 kDa. The apparent molecular mass of rh SCF is approximately 22 kDa in SDS-PAGE under reducing conditions.

Human SCF (aa 1-189) protein, His Tag measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 2-8 ng/mL.

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