The recombinant human IL-6 protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human IL6 (NP_000591.1), (Val 30-Met 212) was expressed, with an initial Met at the N-terminus.

The recombinant human IL6 consists of 184 amino acids and migrates with an apparent molecular mass of 20.3 kDa as estimated in SDS-PAGE under reducing conditions.

Human IL-6 protein
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/ml (100 μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/mL.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.75-3 ng/mL. The specific activity of Recombinant Human IL-6 is approximately 0.35 × 10⁵ IU/μg.

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