The recombinant human IL-33 protein is expressed in E. coli, whose amino acid sequence is derived from C-terminal segment of human IL33 (NP_254274.1), (Ser 112-Thr 270) was expressed with an initial Met at the N-terminus.

The recombinant human IL33 comprises 160 amino acids and predicts a molecular mass of 18 kDa as estimated in SDS-PAGE under reducing conditions.

Human IL-33 protein
1. Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml.
2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells costimulated with anti-CD3. The ED50 for this effect is typically 0.15-0.6 ng/mL.

Abbkine suplies sizes: 5µg, 20µg, 100µg of Human IL-33 proteinfor your choice, more detailed information at https://www.abbkine.com/product/human-il-33-protein-prp100106.

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.