The recombinant human TNF-beta protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human TNFβ (P01374), (Leu 35-Leu 205) was expressed and purified, with an initial Met at the N-terminus.

The recombinant human TNFβ comprises 172 amino acids and predicts a molecular mass of 18.8 kDa as estimated in SDS-PAGE under reducing conditions.

Human TNF-beta protein measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.1-0.5 ng/ml.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.