The success of a ChIP assay is highly dependent on the quality of chromatin prepared. The sonication shearing kit provides buffers to optimise the shearing conditions of your chromatin by sonication.

Isolation of good quality, suitably fragmented chromatin is the most important prerequisite for a successful ChIP assay. Chromatin can be sheared enzymatically or by mechanical methods such as sonication. Shearing by sonication can be advantageous for difficult to lyse cell types, It also provides random sample fragmentation preventing any bias.

The Chromatrap^® Sonication Shearing Kit provides an excellent method for the preparation of high quality, ideally fragmented chromatin for ChIP analysis.

• The perfect solution to optimise the difficult to lyse sample preparations

• Kit supplies all reagents and buffers for up to 10 chromatin preparations

• Allows optimal shearing conditions to generate enough chromatin to perform up to 24 ChIPs using a standard Chromatrap^® ChIP spin column kit or up to 96 IPs using the Chromatrap^® 96 HT microplate kit

Chromatrap Sonication Shearing
Product Code : 500239

Kit Contents: 1.3M Glycine, Hypotonic Buffer, Lysis Buffer, 5M NaCl, 1M NaHCO3, Protease Inhibitor Cocktail (PIC), Proteinase K and Proteinase K Stop Solution.

Porvair Sciences, specialists in the manufacture of microplate products, serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, from sample preparation to high throughput screening via our global distributor network.

Our range includes vacuum manifolds, sealers, evaporators and microtiter plates in all popular styles; deep well and shallow well storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs. We also provide custom microplate products for life science research.

Our vacuum manifolds, essential for 96-well SPE sample preparation, are designed to work with most popular filter plates, including Waters, Millipore, Qiagen, Whatman, GE Healthcare, Varian, Biotage and, of course, Porvair.

For further information, including custom built microplates, reagent reservoirs, vacuum manifold design or microplate handling equipment, please contact us: Tel: +44 1978 666240; Email: int.sales@porvair-sciences.com.