Chromatrap® ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours. The ChIP qPCR assay is a short convenient assay for low chromatin loading with very small slurry volumes (40μls) with no DNA clean up steps prior to qPCR.
By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss. Optimised elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.
The kits have been optimised for 1000ng sample sizes, which allows for better results from smaller sample sizes and greater flexibility as more IP assays can be performed from one sample.
= Designed for qPCR as a downstream process only
= Suitable for low chromatin loadings (1µg-10µg)
= Contains sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitations
= Up to 10 chromatin preparations by sonication
= An enzymatic shearing format is also available
Premium kits containing positive and negative antibody and primer sets to provide you with satisfaction of chromatin preparation are available. A high throughput format is also available allowing for multiple sample type, antibody and gene targets to be tested in parallel.
Chromatrap Premium ChIP qPCR Protein A
Product Code : 500115
Kit Contents: Chromatrap® Protein A spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, Hypotonic Buffer, Elution solution, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K and 1.5ml Collection tubes. The premium kit also includes Positive Antibody Control: Histone H3, Negative Antibody Control: IgG, Forward and Reverse primer set for GAPDH (human promoter region).
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Porvair Sciences, specialists in the manufacture of microplate products, serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, from sample preparation to high throughput screening via our global distributor network.
Our range includes vacuum manifolds, sealers, evaporators and microtiter plates in all popular styles; deep well and shallow well storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs. We also provide custom microplate products for life science research.
Our vacuum manifolds, essential for 96-well SPE sample preparation, are designed to work with most popular filter plates, including Waters, Millipore, Qiagen, Whatman, GE Healthcare, Varian, Biotage and, of course, Porvair.
For further information, including custom built microplates, reagent reservoirs, vacuum manifold design or microplate handling equipment, please contact us: Tel: +44 1978 666240; Email: int.sales@porvair-sciences.com.
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