Product Description

24 Chromatrap®  Protein-A based spin-columns, buffers and reagents for enzymatic shearing of chromatin for ChIP assays.

This Premium kit also includes the following reference standards:

Positive Antibody Control : Histone 3 Rabbit polyclonal
Negative Antibody Control: IgG from Rabbit serum 0.2µg/µl
Forward primer: GAPDH (human promoter region)
Reverse primer: GAPDH (human promoter region)

Kit Contents:

Chromatrap® spin columns 24
Column conditioning buffer 50mls
Wash Buffer 1  50mls
Wash Buffer 2  50mls
Wash Buffer 3 50mls
1.3M Glycine  20mls
Lysis Buffer 10mls
Digestion Buffer 10mls
Enzymatic Stop Solution 200µl
0.1M Elution Solution 3mls
0.1M NaOH  3mls
5M NaCl 500µl
1M NaHCO3 500µl
Hypotonic Buffer  10mls
Shearing Cocktail 100µl
Protease Inhibitor Cocktail (PIC) 100µl
Proteinase K stop solution 100µl
Proteinase K  50µl
1.5ml Collection tube 24

Porvair Sciences, specialists in the manufacture of microplate products, serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, from sample preparation to high throughput screening via our global distributor network.

Our range includes vacuum manifolds, sealers, evaporators and microtiter plates in all popular styles; deep well and shallow well storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs. We also provide custom microplate products for life science research.

Our vacuum manifolds, essential for 96-well SPE sample preparation, are designed to work with most popular filter plates, including Waters, Millipore, Qiagen, Whatman, GE Healthcare, Varian, Biotage and, of course, Porvair.

For further information, including custom built microplates, reagent reservoirs, vacuum manifold design or microplate handling equipment, please contact us: Tel: +44 1978 666240; Email: int.sales@porvair-sciences.com.