Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. LDH is a stable enzyme, present in all cell types, and rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

Abbkine LDH Cytotoxicity Assay Kit provides a simple and easy colorimetric assay for the study of cytotoxicity. The Kit includes LDH Positive Control, Assay Buffer, Lactic Acid Solution, MTT Solution, PES Solution, NAD+ Solution, Triton X-100 (10%).

The LDH Assay Kit(Cytotoxicity) is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the generated color correlates directly with the cell number lysed. In a typical cytotoxicity assay, target cells are cultured with a cytotoxic chemical agent or a cytotoxic cell (NK cell, cytotoxic T cells) to induce target cell death and LDH release. The LDH-containing supernatants are transferred to wells of a new 96-well assay plate and mixed with the LDH Reaction solution. After an incubation of 30 minutes at room temperature, the absorbance at 565nm (A565) is read using a plate reader.

Our LDH Cytotoxicity Detection Kit has below features:
1. Measures cell death in response to chemical compounds or environmental factors. 
2. LDH release from the cytosol into the medium catalyzes a color-forming reaction. 
3. Detect lysis of 10,00-100,0000 cells per well.

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.