One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′-hydroxyl termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.
Abbkine Green Fluorescence TUNEL Apoptosis Detection Kit contains TdT Enzyme, Equilibration Buffer (5X), Label Nucleotide Mix.
Abbkine TUNEL Apoptosis Detection Kit (Green Fluorescence) relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.
The featured
Cell Apoptosis (DNA Fragmentation) Kit has below features:
1. Optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer.
2. Signal can be easily detected at the popular FITC channel (Ex/Em = 490nm/520 nm).
More cell status detection products at
celluar assay kit.
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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.