Species-specific positive probes and negative control probes are needed to run with human or mouse control slides for a quality control checkof the technique and with your sample for Sample or RNA quality control check. ACD provides positive control probes that target specie-specific housekeeping genes and negative control probes that target the bacterial DapB gene.

Careful selection of the right positive control is recommended.  The expected expression level of your target RNA is the key criteria consider as your positive control probe should have similar expression profile.  See the overview page for details.

The same control probes can be used for both RNAscope® 2.5 LS Reagent Kit—BROWN and RNAscope® 2.5 LS Reagent Kit—RED, as both function in Probe Channel 1.  The names for these probes will contain "2.5 LS Control Probes".

For the new RNAscope® 2.5 LS Duplex Assay two types of control probes are available:

Pooled Control probes- Control probes in both C1 and C2 channels

RNAscope® 2.5 LS Duplex Control Probes (PPIB-C1,  Polar2A-C2)- Mm
RNAscope® 2.5 LS Duplex Control Probes (PPIB-C1,  Polar2A-C2)- Hu

50x Control Probes in C2 channel: These probes can be combined with C1 RTU Target probes:

RNAscope® 2.5 LS Negative Control Probe - DapB-C2  (50x)
RNAscope® 2.5 LS Positive Control Probe - Hs-PPIB-C2   (50X)

Sample Images


 

Bio-Techne Corporation (NASDAQ: TECH) is a leading developer and manufacturer of high quality purified proteins:notably cytokines and growth factors, antibodies, immunoassays, as well as biologically active small molecule compounds and ACD's in situ hybridization detection products,which are sold to biomedical researchers and clinical research laboratories; these operations constitute the core Biotechnology Division, headquartered in Minneapolis, Minnesota. The Protein Platforms Division manufactures innovative protein analysis tools under the ProteinSimple brand name that greatly automate western blotting and immunoassay practices. The Diagnostics Division manufactures FDA-regulated controls, calibrators, blood gas and clinical chemistry controls and other reagents for OEM customer and clinical customers. Bio-Techne products are integral components of scientific investigations into biological processes and the nature and progress of specific diseases. They aid in drug discovery efforts and provide the means for accurate clinical tests and diagnoses. With thousands of products in its portfolio, Bio-Techne generated approximately $563 million in net sales in fiscal 2017 and has approximately 1,800 employees worldwide. For more information on Bio-Techne and its brands, please visit www.bio-techne.com.