IPTG IPTG IPTG IPTG
（isopropyl-beta-D-thiogalactopyranoside）
Formula: C9H18O5S
Molecular Weight: 238.3

Store: Store at -20°C
Code No.:ZB079
Package:500g
Price:EUR(€)1500.00
Description
IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. The IPTG is used to induce the expression of cloned genes which are under control of the lac operon. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening.
Applications
•Blue/white colony screening, see Protocol for the Blue/White Colony Screening.
•Induces the expression of cloned genes that are under control of the lac promoter.
Quality Control
Dioxane-free.
Greater than 99% purity confirmed by HPLC.
Functionally tested in blue/white colony screening.
Note
•Preparation of a 23.83 mg/ml (100 mM) stock solution of IPTG in water is recommended.
•For blue/white screening, 0.1 mM final IPTG concentration in LB (Luria Broth) media is recommended.
Protocol for the Blue/White Colony Screening
For individual LB (Luria Broth) agar plates:
1.Pour sterile warm LB agar (about 25 ml) into a Petri dish.
2.Dry opened LB plates at room temperature under UV light for about 30 min.
3.Add 40 µl of the X-Gal Solution (20 mg/ml), ready-to-use.
4.Add 40 µl of 100 mM IPTG Solution, ready-to-use.
5.Spread evenly on the plate with a sterile spatula.
For batch use, add the following directly per 1 ml of the liquid LB agar (kept at about 50°C):
1.1 µl of X-Gal Solution (20 mg/ml), ready-to-use.
2.1 µl of 100 mM IPTG Solution, ready-to-use.
3.Mix well.
4.Pour 25 ml of prepared LB agar into each Petri dish.
5.Dry opened LB plates at room temperature under UV light for about 30 min.

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ShineGene Bio-Technologies, Inc., located in Shanghai, P.R. China, is a bio-company specialized in providing bio- technical services including Gene Synthesis,Peptide Synthesis,Real Time PCR, Fluorogenic Probe Design,Fluorogenic Probe Label,HRP Enzyme Stabilizer,X-gal,IPTG,ProClin300etc. and supplying reagents such as Hotstart Taq polymerase, dNTPs, qPCR mastermix,one step qPCR kits,First Strand cDNA Synthesis Kits,etc. ShineGene also undertake some OEM or OED orders as well as production task.ShineGene works at the cutting edge of development in PCR, quantitative real-time PCR, transposon and RNA technologies. ShineGenes mission is to provide new tools of excellent quality and extreme purity to the scientific community. Therefore research and development is a continuous process in our company.

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