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gDNA/RNA Separate Extraction Kit(Spin-column)
gDNA/RNA Separate Extraction Kit(Spin-column)
Origin of place China
Model 50/100
Supplier BioTeke Corporation
Price $243/$457
Hits 1163
Updated 11/2/2018
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gDNA/RNA Separate Extraction Kit(Spin-column)
 

Cat.# Preps Prices(USD)
RP5501 50 243
RP5502 100 457



Principle
Biological samples are first lysed and homogenized in the presence of a highly denaturing guanidine-thiocyanate–containing buffer, which immediately inactivates RNases to ensure intact RNA. Ethanol is added to provide appropriate binding conditions, and mixture is then applied to the first spin-column, where genomic DNA binds to the membrane, then add filtrate to the second spin-column, and total RNA binds to this membrane. Finally, contaminants are efficiently washed away separately. High-quality gDNA and total RNA are then eluted in 30-100 μl water from separate spin-column.

Features
*Stability, comparable RNA yield with high quality absorbing membrane.
*High-purity, specifically membrane absorption and washing for removing protein and other debris.
*No protein contamination.
 

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