RNApure High-purity Total RNA Rapid Extraction Kit(Spin-column)

SKU	Preps	Price（USD）
RP1201	20	70
RP1202	50	130

Principle

The RNApure procedure represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica membrane with the speed of microspin technology. A specialized high-salt buffer system allows up to 100 μg of RNA longer than 200 bases to bind to the RNApure silica membrane. 
Biological samples are first lysed and homogenized in the presence of a highly denaturing guanidine-thiocyanate–containing buffer, which immediately inactivates RNases to ensure intact RNA. Ethanol is added to provide appropriate binding conditions, and the sample is then applied to an RNApure Mini spin-column, where the total RNA binds to the membrane and contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100 μl water.  

Features

◆ Stability, comparable RNA yield with high quality absorbing membrane.
◆ High-purity, specifically membrane absorption and washing for removing protein and other debris.  

Expected yields of RNA per mg of tissue or 1 x 10⁶ cultured cells

Liver and spleen, 6-10 µg
Kidney, 3-4 µg
Skeletal muscles and brain, 1-1.5 µg
Placenta, 1-4 µg
Epithelial cells (1 x 10⁶ cultured cells), 8-15 µg
Fibroblasts, (1 x10⁶cultured cells) 5-7 µg

BIOTEKE is a leading biotechnology company in China since 2005,
Our products nucleic acid extraction/purification as well as related essential lab instruments.
Please check our website here: http://english.bioteke.cn/

Our main product portfolio includes but not limits the following items,
Reagents/kits for molecular biological research uses,
DNA/RNA/plasmid extraction kits, PCR/RT-PCR/qPCR products, DNA/RNA stains/markers.

Automated Nucleic Acid Extraction systems, together with it's magnetic beeds kits;
For extracting DNA/RNA from various samples(cfDNA, blood, tissue/cells, plants, plasmid, etc) ,
32 samples or 96 samples in one time in 30min,
The cost for one samples is less than 4USD for our clients.

Micro Spectrophotometer ND5000,
0.5-2uL sample is needed, 4s for 1 test, to test the concentration of DNA/RNA/proteins.

Storage tubes for blood RNA, cfDNA, Urine DNA, swab/saliva DNA, stool DNA samples.

samples can be transported for more than one week, or for long term storage in low temprature.
widely used in clinical sample collections.Trial samples are available.

Our R