Detect the type of DNA damage with FLARE^TM Assays

The FLARE^TM Assay (Fragment Length Analysis using Repair Enzymes) provides the ability to detect DNA damage in single cells using a variety of DNA repair enzymes in conjunction with CometAssay^TM single cell gel electrophoresis kit. To assess the type of DNA damage induced by a putative mutagen, drug, or treatment regimen, cells are harvested after treatment and immobilized in a layer of low melting point agarose on the FLARE Slide. The cells are gently lysed and subsequently incubated with a kit-specific enzyme which recognizes a particular type of DNA damage (see below) and cleaves the DNA. After treatment with alkali to denature the DNA, the samples are submitted to neutral gel electrophoresis, and stained using SYBR(r) Green I.

Denatured, cleaved DNA fragments migrate out of the cell during electrophoresis, while undamaged, supercoiled DNA remains within the confines of the nuclear cell membrane. The extent of the DNA damage can be assessed qualitatively, or quantitatively using image analysis software to calculate tail length and tail moment.

 Features:

• FLARE Slides allow direct application of low melting point agarose without base layers or pretreatment for three samples
• Hydrophobic barrier allows easy application of cells directly to slide and treatment with enzyme
• Base specific DNA damage detection in a convenient format

Available reagents:

• Kits (-FK) provide the enzyme, reagents, and FLARE slides for 75 samples
• Modules (-FM) provide enzyme and reagents for >100 samples

Catalog # Assay Type Damage Recognized Damage 4040-100-FK 4040-100-FM E.coli Fpg 8-oxoguanine, DNA containing formamidopyrimidine moieties Oxidative Damage 4045-01K-FK 4045-01K-FM E.coli Endonuclease III Thymine Glycol, 5,6-dihydrothymine, urea, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil, alloxan, 5-hydroxy-6-hydrouracil, uracil glycol, 5-hydroxy-5-methylhydantoin, 5-hydroxycytosine,5-hydroxyuracil, methyltartonylurea, thymine ring saturated or fragmentation product 4130-100-FK 4130-100-FM Human OGG1 8-oxoguanine, DNA containing formamidopyrimidine moieties 4055-100-FK 4055-100-FM T4-PDG Cis-syn isomers of cyclobutane pyrimidine dimers UV Damage 4065-100-FK 4065-100-FM Chorella virus-PDG Cis-syn and trans-syn isomers of cyclobutane pyrimidine dimers 4100-100-FK 4100-100-FM S. pombe UVDE CyclobuCyclobutane pyrimidine dimers, (6-4) photoproducts Download a Technical Presentation about FLARETM& Comet Assay

FLARE Assays:Other consumables

Catalog #	Component	Pack Size
4256-010-CC	Control cells	10 assays
3950-075-02	FLARE slides	25
3950-300-02	FLARE slides	100
3950-040-01	25X FLARE buffer 1	40 ml
3951-040-01	25X FLARE buffer 2	40 ml
3950-100-04	FLARE Buffer BSA Additive	100 ul
3950-075-SP	FLARE Sample Prep	75 samples
4252-040-02	CometSlide Rack System	1 rack
3950-100-05	FLARE Buffer Cation Solution	100 ul

FLARE, CometAssay and CometSlide are trademarks of Trevigen, Inc.

AMSBIO’s mission is to be a profitable premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines. This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.

Formed in 1987 by Sandy Allan and Alex Sim the company’s first offices were in Madrid, Spain. Over the following three years further offices were opened in Italy, Switzerland and the UK. During the formative years of existence AMS helped launch the successful sales campaigns of Stratagene and Invitrogen providing them with an important foothold from which to develop their global brand expansion.