Sequencing Grade Trypsin

Catalog Number:SRT0201

CAS:9002-07-7
EC:3.4.21.4

Storage temperature: 2-8℃

Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli 

Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin

 

1.DESCRIPTION

YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant  trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

2.MAIN FEATURES

Source	Recombinant E. coli
Appearance	White or off white, or yellowish powder
Specific activity	≥4500 USP units/mg pro.
SDS-PAGE	Single major band
Molecular Weight(SDS-PAGE)	24.0±2.4 kDa
Purity(HPLC)	≥95%

 

3.RECOMMEND USAGE

It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO₃ or ph7.0-8.0 buffer solution. 1 mM CaCl₂ is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.

 

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃. 

It is no activity loss after 5 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

5.PRODUCT ADVANTAGE

• Sequence grade recombinant protein: high specificity,good stability.
• Animal origin free: sequencing grade modified trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
• Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
• High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
• Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
• Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
• Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

 

6.PRODUCT USAGE

The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.

 

7.RELATED PRODUCTS

Recombinant enterokinase

Recombinant V8 protease

Sequencing grade chymotrypsin

Sequencing grade carboxypeptidase B

Shanghai Yaxin Biotechnology Co. Ltd. is a biotech company founded in 2008 that focuses on the research and the production of recombinant proteins. Shanghai YaxinBio,located in Shanghai Juke Biotech Park, is ISO 9001 registered company since 2009.


Our main products are Animal Origin Free recombinant carboxypeptidase B and recombinant trypsin, which are of high importance for the production of human recombinant insulin.YaxinBio recombinant carboxypeptidase B and recombinant trypsin have no trace of other contaminating enzyme activities or enzyme inhibitors. They have been successfully used in the production process of recombinant insulin by several companies. Recombinant Trypsin can also be used in the process of cell culture.


Shanghai YaxinBio continuously strives to bring to its worldwide customers the best recombinant protein products. We will continue strengthen our product management and our internal system to meet the demands of biotech companies and scientists in the field of life sciences.