QSER1 protein contains two high conserved domains found not only in QSER1 but also in other protein products. This property was conserved from the human QSER1 to the Coelacanth QSER1. Multiple conserved nuclear localization signals were also predicted within the QSER1 protein by pSORT. Predictions of the QSER1 protein structure indicate that the protein contains many alpha helices. NCBI cBLAST predicted structural similarity between the QSER1 protein and the Schizosaccharomyces pombe (fission yeast) RNA Polymerase II A chain. The two regions of similarity occur between amino acids 56-194 and 322-546. This first region (56-194) is a regulatory region in both the human and yeast RNA Polymerase II containing multiple repeats of the sequence YSPTSPSYS.
Human Glutamine and serine-rich protein 1 (QSER1) ELISA Kit employs a two-site sandwich ELISA to quantitate QSER1 in samples. An antibody specific for QSER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQSER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QSER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QSER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Glutamine and serine-rich protein 1 (QSER1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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