DEGS1 encodes a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. Sphingolipid delta contains three His-containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It is predicted to be a multiple membrane-spanning protein localized to the endoplasmic reticulum. Overexpression of DEGS1 inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor.
Chicken Sphingolipid delta (DEGS1) ELISA Kit employs a two-site sandwich ELISA to quantitate DEGS1 in samples. An antibody specific for DEGS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDEGS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEGS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEGS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Chicken Sphingolipid delta (DEGS1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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