DNA2 interacted with mitochondrial DNA polymerase-gamma and significantly stimulated its polymerase activity. DNA2 and flap endonuclease-1 (FEN1) synergistically processed intermediate 5-prime flap structures occurring in DNA replication and long-patch base excision repair (LP-BER) in mitochondria.The deduced 1,076-amino acid protein has a putative ATP/GTP-binding site motif A and a predicted transmembrane domain. Northern blot analysis detected low expression in KG-1 and HeLa cells and in thymus, but not in any other human tissue examined.Yeast Dna2 helicase is a DNA-dependent ATPase that unwinds duplex DNA to generate single-stranded DNA, which then acts as a template for DNA polymerization during DNA replication.
Chicken DNA2-like helicase (DNA2) ELISA Kit employs a two-site sandwich ELISA to quantitate DNA2 in samples. An antibody specific for DNA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Chicken DNA2-like helicase (DNA2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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