Mediator of RNA polymerase II transcription subunit 17 (MED17) is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Chicken Mediator of RNA polymerase II transcription subunit 17 (MED17) ELISA Kit employs a two-site sandwich ELISA to quantitate MED17 in samples. An antibody specific for MED17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Chicken Mediator of RNA polymerase II transcription subunit 17 (MED17) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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