Cyclooxygenase (COX) enzymes catalyze the synthesis of prostaglandins (PGs) from arachidonic acid. There are two isoforms of COX, COX-1 and COX-2. While COX-1 is expressed constitutively and appears to be responsible for the production of prostaglandins (PGs) that control normal physiologic functions, expression of COX-2 is induced by various inflammatory and mitogenic stimuli such as cytokines, growth factors or tumor promoters. Numerous experimental studies suggest a relationship between COX-2 expression and carcinogenesis. For example, increased amounts of COX-2 have been observed in breast cancers that overexpress HER-2/neu. Furthermore, treatment with selective inhibitors of COX-2 reduced the formation of tongue, esophagal, intestinal, breast, skin, lung, and bladder tumors in experimental animals.
Chicken Prostaglandin G/H synthase 2 (PTGS2) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGS2 in samples. An antibody specific for PTGS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Chicken Prostaglandin G/H synthase 2 (PTGS2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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