The troponin C protein is the calcium-binding subunit of the troponin complex. It occurs in 2 distinct isoforms: fast skeletal troponin C, which is expressed exclusively in fast-twitch skeletal muscle, and slow/cardiac troponin C, which is expressed in both cardiac and slow-twitch skeletal muscle. Gahlmann and Kedes (1990) described the structure and sequence of the gene for fast-twitch troponin C.By PCR-based analysis of a monochromosomal hybrid panel followed by analysis of the Genebridge 4 radiation hybrid panel, Townsend et al. (1997) mapped the TNNC2 gene to chromosome 20. Tiso et al. (1997) independently mapped the TNNC2 gene to 20q12-q13.11 using PCR of radiation hybrids.
Chicken Troponin C, skeletal muscle (TNNC2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNC2 in samples. An antibody specific for TNNC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Chicken Troponin C, skeletal muscle (TNNC2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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