Reactive oxygen species (ROS) are chemically reactive chemical species containing oxygen. Examples include peroxides, superoxide, hydroxyl radical, and singlet oxygen. In a biological context, ROS are formed as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation.
Canine Reactive OXyen Species (ROS) ELISA Kit employs a two-site sandwich ELISA to quantitate ROS in samples. An antibody specific for ROS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyROS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ROS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ROS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Canine Reactive OXyen Species (ROS) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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