Angiotensin(1-7) (Ang1-7) acting at the level of the rostral ventrolateral medulla (RVLM) affects arterial pressure. Astroglia are a plausible cellular target of Ang1-7 in RVLM. Astrocytic responsiveness to Ang1-7 is reduced in SHR. Ang1-7 modulates astrocytic signalling which in vivo may affect local metabolism and microcirculation, resulting in changes in activity of RVLM pre-sympathetic neurones and hence blood pressure.Ang1-7 is an important component of the central renin-angiotensin system and affects haemodynamics when injected into the RVLM. Effect of Ang1-7 could be blocked by co-application of its antagonist, A779 (200 nM) but not losartan (1 μM). non-CA neurones and glia in RVLM are the primary targets for Ang1-7 and its effect on [Ca2+]i in non-CA neurones is enhanced in SHR.
Canine Angiotension 1-7 (Ang1-7) ELISA Kit employs a two-site sandwich ELISA to quantitate Ang1-7 in samples. An antibody specific for Ang1-7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAng1-7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Ang1-7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ang1-7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Canine Angiotension 1-7 (Ang1-7) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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