NADH-ubiquinone oxidoreductase chain 6 is a protein that in humans is encoded by the mitochondrial MT-ND6 gene. The ND6 protein is a subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variations in the MT-ND6 gene are associated with Leigh's syndrome, Leber's hereditary optic neuropathy (LHON) and dystonia.
Canine NADH-ubiquinone oxidoreductase chain 6 (MT-ND6) ELISA Kit employs a two-site sandwich ELISA to quantitate MT-ND6 in samples. An antibody specific for MT-ND6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-ND6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-ND6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-ND6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Canine NADH-ubiquinone oxidoreductase chain 6 (MT-ND6) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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