Plasmin is an important enzyme (EC 3.4.21.7) present in blood that degrades many blood plasma proteins, most notably, fibrin clots. The degradation of fibrin is termed fibrinolysis. Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between Arg-560 and Val-561, which is mediated by urokinase and tissue plasminogen activator. The main function of plasmin is to dissolve fibrin blood clots. Plasmin, like trypsin, belongs to the family of serine proteases.Plasmin is a serine protease that is released as plasminogen from the liver into the circulation and activated by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), and factor XII (Hageman factor).
Canine Plasminogen (PLG) ELISA Kit employs a two-site sandwich ELISA to quantitate PLG in samples. An antibody specific for PLG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Canine Plasminogen (PLG) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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